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CH02 peptide promotes ex vivo expansion of umbilical cord blood-derived CD34 + hematopoietic stem/progenitor cells.

ABSTRACT: Umbilical cord blood (UCB) is an advantageous source for hematopoietic stem/progenitor cell (HSPC) transplantation, yet the current strategies for large-scale and cost-effective UCB-HSPC preparation are still unavailable. To overcome these obstacles, we systematically evaluate the feasibility of our newly identified CH02 peptide for ex vivo expansion of CD34 + UCB-HSPCs. We herein report that the CH02 peptide is specifically enriched in HSPC proliferation via activating the FLT3 signaling. Notably, the CH02-based cocktails are adequate for boosting 12-fold ex vivo expansion of UCB-HSPCs. Meanwhile, CH02-preconditioned UCB-HSPCs manifest preferable efficacy upon wound healing in diabetic mice via bidirectional orchestration of proinflammatory and anti-inflammatory factors. Together, our data indicate the advantages of the CH02-based strategy for ex vivo expansion of CD34 + UCB-HSPCs, which will provide new strategies for further development of large-scale HSPC preparation for clinical purposes.

SUBMITTER: Yang Y 

PROVIDER: S-EPMC10577473 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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CH02 peptide promotes <i>ex vivo</i> expansion of umbilical cord blood-derived CD34 <sup>+</sup> hematopoietic stem/progenitor cells.

Yang Yiqi Y   Zhang Bihui B   Xie Junye J   Li Jingsheng J   Liu Jia J   Liu Rongzhan R   Zhang Linhao L   Zhang Jinting J   Su Zijian Z   Li Fu F   Zhang Leisheng L   Hong An A   Chen Xiaojia X  

Acta biochimica et biophysica Sinica 20231001 10

Umbilical cord blood (UCB) is an advantageous source for hematopoietic stem/progenitor cell (HSPC) transplantation, yet the current strategies for large-scale and cost-effective UCB-HSPC preparation are still unavailable. To overcome these obstacles, we systematically evaluate the feasibility of our newly identified CH02 peptide for <i>ex vivo</i> expansion of CD34 <sup>+</sup> UCB-HSPCs. We herein report that the CH02 peptide is specifically enriched in HSPC proliferation via activating the FLT  ...[more]

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