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Staphylococcus aureus sacculus mediates activities of M23 hydrolases.

ABSTRACT: Peptidoglycan, a gigadalton polymer, functions as the scaffold for bacterial cell walls and provides cell integrity. Peptidoglycan is remodelled by a large and diverse group of peptidoglycan hydrolases, which control bacterial cell growth and division. Over the years, many studies have focused on these enzymes, but knowledge on their action within peptidoglycan mesh from a molecular basis is scarce. Here, we provide structural insights into the interaction between short peptidoglycan fragments and the entire sacculus with two evolutionarily related peptidases of the M23 family, lysostaphin and LytM. Through nuclear magnetic resonance, mass spectrometry, information-driven modelling, site-directed mutagenesis and biochemical approaches, we propose a model in which peptidoglycan cross-linking affects the activity, selectivity and specificity of these two structurally related enzymes differently.

SUBMITTER: Razew A

PROVIDER: S-EPMC10593780 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Staphylococcus aureus sacculus mediates activities of M23 hydrolases.

Razew Alicja A Laguri Cedric C Vallet Alicia A Bougault Catherine C Kaus-Drobek Magdalena M Sabala Izabela I Simorre Jean-Pierre JP

Nature communications 20231023 1

Peptidoglycan, a gigadalton polymer, functions as the scaffold for bacterial cell walls and provides cell integrity. Peptidoglycan is remodelled by a large and diverse group of peptidoglycan hydrolases, which control bacterial cell growth and division. Over the years, many studies have focused on these enzymes, but knowledge on their action within peptidoglycan mesh from a molecular basis is scarce. Here, we provide structural insights into the interaction between short peptidoglycan fragments a ...[more]

PMID: 37872144

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