Ontology highlight
ABSTRACT:
SUBMITTER: Yu CP
PROVIDER: S-EPMC1065124 | biostudies-literature | 2005 Mar
REPOSITORIES: biostudies-literature
Yu Chang-Ping CP Ahuja Rajiv R Sayler Gary G Chu Kung-Hui KH
Applied and environmental microbiology 20050301 3
A quantitative fingerprinting method, called the real-time terminal restriction fragment length polymorphism (real-time-t-RFLP) assay, was developed for simultaneous determination of microbial diversity and abundance within a complex community. The real-time-t-RFLP assay was developed by incorporating the quantitative feature of real-time PCR and the fingerprinting feature of t-RFLP analysis. The assay was validated by using a model microbial community containing three pure strains, an Escherich ...[more]