Generation and ex vivo culture of murine and human pancreatic ductal adenocarcinoma tissue slice explants.
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ABSTRACT:
SUBMITTER: Decker-Farrell AR
PROVIDER: S-EPMC10682255 | biostudies-literature | 2023 Nov
REPOSITORIES: biostudies-literature
Publications
Generation and ex vivo culture of murine and human pancreatic ductal adenocarcinoma tissue slice explants.
Decker-Farrell Amanda R AR Ma Alice A Li Fangda F Muir Alexander A Olive Kenneth P KP
STAR protocols 20231111 4
Traditional 2D/3D co-culture models typically do not reflect the cellular heterogeneity of pancreatic ductal adenocarcinoma (PDAC) tumors, while in vivo models can be slow and ill-suited to mechanistic investigations. Here, we present a protocol for culturing murine PDAC explants and a corresponding human PDAC model using tissue slice explants. We describe steps for sponge production, preparation of media and materials, tissue collection, and sectioning. We then detail procedures for explant pla ...[more]
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