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Vascular beds maintain pancreatic tumour explants for ex vivo drug screening.


ABSTRACT: Our understanding of cancer progression or response to therapies would benefit from benchtop, tissue-level assays that preserve the biology and anatomy of human tumours ex vivo. We present a methodology for maintaining patient tumour samples ex vivo for the purpose of drug testing in a clinical setting. The harvested tumour biopsy, excised from mice or patients, is integrated into a support tissue that includes stroma and vasculature. This support tissue preserves tumour histoarchitecture and relevant expression profiles, and tumour tissues cultured using this system display different sensitivities to chemotherapeutics compared with tumour explants with no supporting tissue. The methodology is more rapid than patient-derived xenograft models, easy to implement, and amenable to high-throughput assays, making it an attractive tool for in vitro drug screening or for the guidance of patient-specific chemotherapies.

SUBMITTER: Bazou D 

PROVIDER: S-EPMC5711628 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Vascular beds maintain pancreatic tumour explants for ex vivo drug screening.

Bazou Despina D   Maimon Nir N   Gruionu Gabriel G   Grahovac Jelena J   Seano Giorgio G   Liu Hao H   Evans Conor L CL   Munn Lance L LL  

Journal of tissue engineering and regenerative medicine 20170912 1


Our understanding of cancer progression or response to therapies would benefit from benchtop, tissue-level assays that preserve the biology and anatomy of human tumours ex vivo. We present a methodology for maintaining patient tumour samples ex vivo for the purpose of drug testing in a clinical setting. The harvested tumour biopsy, excised from mice or patients, is integrated into a support tissue that includes stroma and vasculature. This support tissue preserves tumour histoarchitecture and re  ...[more]

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