Ontology highlight
ABSTRACT:
SUBMITTER: Lin GH
PROVIDER: S-EPMC107026 | biostudies-literature | 1998 Mar
REPOSITORIES: biostudies-literature
Journal of bacteriology 19980301 5
A fengycin synthetase gene, fenB, has been cloned and sequenced. The protein (FenB) encoded by this gene has a predicted molecular mass of 143.6 kDa. This protein was overexpressed in Escherichia coli and was purified to near homogeneity by affinity chromatography. Experimental results indicated that the recombinant FenB has a substrate specificity toward isoleucine with an optimum temperature of 25 degrees C, an optimum pH of 4.5, a Km value of 922 microM, and a turnover number of 236 s(-1). Fe ...[more]