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Molecular cloning and characterization of fengycin synthetase gene fenB from Bacillus subtilis.

ABSTRACT: A fengycin synthetase gene, fenB, has been cloned and sequenced. The protein (FenB) encoded by this gene has a predicted molecular mass of 143.6 kDa. This protein was overexpressed in Escherichia coli and was purified to near homogeneity by affinity chromatography. Experimental results indicated that the recombinant FenB has a substrate specificity toward isoleucine with an optimum temperature of 25 degrees C, an optimum pH of 4.5, a Km value of 922 microM, and a turnover number of 236 s(-1). FenB also consists of a thioesterase domain, suggesting that this protein may be involved in the activation of the last amino acid of fengycin.

SUBMITTER: Lin GH

PROVIDER: S-EPMC107026 | biostudies-literature | 1998 Mar

REPOSITORIES: biostudies-literature

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Molecular cloning and characterization of fengycin synthetase gene fenB from Bacillus subtilis.

Lin G H GH Chen C L CL Tschen J S JS Tsay S S SS Chang Y S YS Liu S T ST

Journal of bacteriology 19980301 5

A fengycin synthetase gene, fenB, has been cloned and sequenced. The protein (FenB) encoded by this gene has a predicted molecular mass of 143.6 kDa. This protein was overexpressed in Escherichia coli and was purified to near homogeneity by affinity chromatography. Experimental results indicated that the recombinant FenB has a substrate specificity toward isoleucine with an optimum temperature of 25 degrees C, an optimum pH of 4.5, a Km value of 922 microM, and a turnover number of 236 s(-1). Fe ...[more]

PMID: 9495777

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