Ontology highlight
ABSTRACT:
SUBMITTER: Courtney CR
PROVIDER: S-EPMC3415477 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Courtney Colleen R CR Cozy Loralyn M LM Kearns Daniel B DB
Journal of bacteriology 20120622 17
The structure of the Gram-positive flagellum is poorly understood, and Bacillus subtilis encodes three proteins homologous to the flagellar hook protein from Salmonella enterica. Here we generated a modified B. subtilis hook protein that could be fluorescently stained using a cysteine-reactive dye. We used the fluorescently labeled hook to demonstrate that FlgE is the hook structural protein and that FliK regulated hook length. We further demonstrate that two proteins of unknown function, FlhO a ...[more]