Ontology highlight
ABSTRACT:
SUBMITTER: Sun C
PROVIDER: S-EPMC10722395 | biostudies-literature | 2023 Dec
REPOSITORIES: biostudies-literature
Sun Chixiang C Hu Baodong B Li Yanchun Y Wu Zhimeng Z Zhou Jingwen J Li Jianghua J Chen Jian J Du Guocheng G Zhao Xinrui X
Synthetic and systems biotechnology 20231125 4
Deoxycholic acid (DCA) has been authorized by the Federal Drug Agency for cosmetic reduction of redundant submental fat. The hydroxylated product (6β-OH DCA) was developed to improve the solubility and pharmaceutic properties of DCA for further applications. Herein, a combinatorial catalytic strategy was applied to construct a powerful Cytochrome P450 biocatalyst (CYP107D1, OleP) to convert DCA to 6β-OH DCA. Firstly, the weak expression of OleP was significantly improved using pRSFDuet-1 plasmid ...[more]