Unknown

Dataset Information

0

β3 Adrenoceptor Agonism Prevents Hyperoxia-Induced Colonic Alterations.


ABSTRACT: Oxygen level is a key regulator of organogenesis and its modification in postnatal life alters the maturation process of organs, including the intestine, which do not completely develop in utero. The β3-adrenoreceptor (β3-AR) is expressed in the colon and has an oxygen-dependent regulatory mechanism. This study shows the effects of the β3-AR agonist BRL37344 in a neonatal model of hyperoxia-driven colonic injury. For the first 14 days after birth, Sprague-Dawley rat pups were exposed to ambient oxygen levels (21%) or hyperoxia (85%) and treated daily with BRL37344 at 1, 3, 6 mg/kg or untreated. At the end of day 14, proximal colon samples were collected for analysis. Hyperoxia deeply influences the proximal colon development by reducing β3-AR-expressing cells (27%), colonic length (26%) and mucin production (47%), and altering the neuronal chemical coding in the myenteric plexus without changes in the neuron number. The administration of BRL37344 at 3 mg/kg, but not at 1 mg/kg, significantly prevented these alterations. Conversely, it was ineffective in preventing hyperoxia-induced body weight loss. BRL37344 at 6 mg/kg was toxic. These findings pave the way for β3-AR pharmacological targeting as a therapeutic option for diseases caused by hyperoxia-impaired development, typical prematurity disorders.

SUBMITTER: Filippi L 

PROVIDER: S-EPMC10741994 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications


Oxygen level is a key regulator of organogenesis and its modification in postnatal life alters the maturation process of organs, including the intestine, which do not completely develop in utero. The β3-adrenoreceptor (β3-AR) is expressed in the colon and has an oxygen-dependent regulatory mechanism. This study shows the effects of the β3-AR agonist BRL37344 in a neonatal model of hyperoxia-driven colonic injury. For the first 14 days after birth, Sprague-Dawley rat pups were exposed to ambient  ...[more]

Similar Datasets

| S-EPMC10861817 | biostudies-literature
| S-EPMC11361380 | biostudies-literature
| S-EPMC3202580 | biostudies-literature
| S-EPMC7138611 | biostudies-literature
| S-EPMC4461769 | biostudies-literature
| S-EPMC9029465 | biostudies-literature
| S-EPMC4556897 | biostudies-literature
| S-EPMC5295490 | biostudies-literature
| S-EPMC9808120 | biostudies-literature
| S-EPMC3761642 | biostudies-literature