Ontology highlight
ABSTRACT:
SUBMITTER: Meydan S
PROVIDER: S-EPMC10755677 | biostudies-literature | 2023 Nov
REPOSITORIES: biostudies-literature
Meydan Sezen S Barros Géssica C GC Simões Vanessa V Harley Lana L Cizubu Blanche K BK Guydosh Nicholas R NR Silva Gustavo M GM
Cell reports 20231102 11
Oxidative stress causes K63-linked ubiquitination of ribosomes by the E2 ubiquitin conjugase Rad6. How Rad6-mediated ubiquitination of ribosomes affects translation, however, is unclear. We therefore perform Ribo-seq and Disome-seq in Saccharomyces cerevisiae and show that oxidative stress causes ribosome pausing at specific amino acid motifs, which also leads to ribosome collisions. However, these redox-pausing signatures are lost in the absence of Rad6 and do not depend on the ribosome-associa ...[more]