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Novel polyomavirus detected in the feces of a chimpanzee by nested broad-spectrum PCR.

ABSTRACT: In order to screen for new polyomaviruses in samples derived from various animal species, degenerated PCR primer pairs were constructed. By using a nested PCR protocol, the sensitive detection of nine different polyomavirus genomes was demonstrated. The screening of field samples revealed the presence of a new polyomavirus, tentatively designated chimpanzee polyomavirus (ChPyV), in the feces of a juvenile chimpanzee (Pan troglodytes). Analysis of the region encoding the major capsid protein VP1 revealed a unique insertion in the EF loop of the protein and showed that ChPyV is a distinct virus related to the monkey polyomavirus B-lymphotropic polyomavirus and the human polyomavirus JC polyomavirus.

SUBMITTER: Johne R

PROVIDER: S-EPMC1075742 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature

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Novel polyomavirus detected in the feces of a chimpanzee by nested broad-spectrum PCR.

Johne Reimar R Enderlein Dirk D Nieper Hermann H Müller Hermann H

Journal of virology 20050301 6

In order to screen for new polyomaviruses in samples derived from various animal species, degenerated PCR primer pairs were constructed. By using a nested PCR protocol, the sensitive detection of nine different polyomavirus genomes was demonstrated. The screening of field samples revealed the presence of a new polyomavirus, tentatively designated chimpanzee polyomavirus (ChPyV), in the feces of a juvenile chimpanzee (Pan troglodytes). Analysis of the region encoding the major capsid protein VP1 ...[more]

PMID: 15731285

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