Ontology highlight
ABSTRACT:
SUBMITTER: Kleter B
PROVIDER: S-EPMC1866345 | biostudies-literature | 1998 Dec
REPOSITORIES: biostudies-literature
Kleter B B van Doorn L J LJ ter Schegget J J Schrauwen L L van Krimpen K K Burger M M ter Harmsel B B Quint W W
The American journal of pathology 19981201 6
A novel set of polymerase chain reaction (PCR) primers, designated SPF1 and SPF2 and located in the L1 region, was developed for universal detection of human papillomavirus (HPV). A short PCR fragment (SPF) of only 65 pb was synthesized. SPF amplimers were detected in a microtiter-based hybridization system, using a mixture of oligonucleotide probes. The SPF system allowed detection of at least 43 different HPV genotypes. The clinical performance of the novel SPF system was assessed in three dif ...[more]