A dual-light reporter system to determine the efficiency of protein-protein interactions in mammalian cells.

ABSTRACT: Methods for determining protein-protein interactions in mammalian cells typically rely on single reporter functions and are susceptible to variations between samples particularly in regard to levels of transcription, processing and translation. A method has been developed for determining protein-protein interactions in mammalian cells, which bypasses these variables confounding single reporter assays. The approach utilizes two units of gene expression linked to reporter functions that are interposed by a deactivation-activation unit in such a way that the downstream expression unit is switched off. Hence upstream expression occurs regardless of protein-protein interaction, leading to the production of the upstream reporter. In the event of protein-protein interactions, the downstream expre

SUBMITTER: Nasim MT 

PROVIDER: S-EPMC1075926 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature