Unknown

Dataset Information

0

Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.


ABSTRACT: Studying the functions of S-palmitoylated proteins in cells can be challenging due to the membrane targeting property and dynamic nature of protein S-palmitoylation. New strategies are therefore needed to specifically capture S-palmitoylated protein complexes in cellular membranes for dissecting their functions in vivo. Here we present a bifunctional fatty acid chemical reporter, x-alk-16, which contains an alkyne and a diazirine, for metabolic labeling of S-palmitoylated proteins and photo-cross-linking of their involved protein complexes in mammalian cells. We demonstrate that x-alk-16 can be metabolically incorporated into known S-palmitoylated proteins such as H-Ras and IFITM3, a potent antiviral protein, and induce covalent cross-linking of IFITM3 oligomerization as well as its specific interactions with other membrane proteins upon in-cell photoactivation. Moreover, integration of x-alk-16-induced photo-cross-linking with label-free quantitative proteomics allows identification of new IFITM3 interacting proteins.

SUBMITTER: Peng T 

PROVIDER: S-EPMC4303541 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.

Peng Tao T   Hang Howard C HC  

Journal of the American Chemical Society 20150112 2


Studying the functions of S-palmitoylated proteins in cells can be challenging due to the membrane targeting property and dynamic nature of protein S-palmitoylation. New strategies are therefore needed to specifically capture S-palmitoylated protein complexes in cellular membranes for dissecting their functions in vivo. Here we present a bifunctional fatty acid chemical reporter, x-alk-16, which contains an alkyne and a diazirine, for metabolic labeling of S-palmitoylated proteins and photo-cros  ...[more]

Similar Datasets

| S-EPMC8551652 | biostudies-literature
2023-01-01 | GSE153619 | GEO
| S-EPMC8470630 | biostudies-literature
| S-EPMC1075926 | biostudies-literature
| S-EPMC3246509 | biostudies-literature
2022-02-15 | PXD028244 | Pride
| S-EPMC5798302 | biostudies-literature
| S-EPMC8147873 | biostudies-literature
| S-EPMC3365931 | biostudies-literature
| S-EPMC4764564 | biostudies-literature