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Development of single-cell transcriptomic atlas of human plasmacytoid dendritic cells from people with HIV-1.

ABSTRACT: Many immunological treatment strategies for reducing the HIV-1 reservoir and enhancing adaptive immunity aim at activating the human plasmacytoid dendritic cells (pDCs). Here, we present a protocol for pDC enrichment, single-cell analysis, and development of a pDC transcriptomic database from healthy individuals and people with HIV-1 before and after Toll-like receptor 9 agonist treatment. For complete details on the use and execution of this protocol, please refer to Cham et al.¹.

SUBMITTER: Cham LB

PROVIDER: S-EPMC10777061 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Development of single-cell transcriptomic atlas of human plasmacytoid dendritic cells from people with HIV-1.

Cham Lamin B LB Lin Lin L Tolstrup Martin M Søgaard Ole S OS

STAR protocols 20231221 1

Many immunological treatment strategies for reducing the HIV-1 reservoir and enhancing adaptive immunity aim at activating the human plasmacytoid dendritic cells (pDCs). Here, we present a protocol for pDC enrichment, single-cell analysis, and development of a pDC transcriptomic database from healthy individuals and people with HIV-1 before and after Toll-like receptor 9 agonist treatment. For complete details on the use and execution of this protocol, please refer to Cham et al.<sup>1</sup>. ...[more]

PMID: 38133956

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