Unknown

Dataset Information

0

The Arcanobacterium (Actinomyces) pyogenes plasmid pAP1 is a member of the pIJ101/pJV1 family of rolling circle replication plasmids.


ABSTRACT: The 2.4-kb plasmid pAP1 from Arcanobacterium (Actinomyces) pyogenes had sequence similarity within the putative replication protein and double-stranded origin with the pIJ101/pJV1 family of plasmids. pJGS84, a derivative of pAP1 containing a kanamycin resistance gene, was able to replicate in Escherichia coli and Corynebacterium pseudotuberculosis, as well as in A. pyogenes. Detection of single-stranded DNA intermediates of pJGS84 replication suggested that this plasmid replicates by the rolling circle mechanism.

SUBMITTER: Billington SJ 

PROVIDER: S-EPMC107828 | biostudies-literature | 1998 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

The Arcanobacterium (Actinomyces) pyogenes plasmid pAP1 is a member of the pIJ101/pJV1 family of rolling circle replication plasmids.

Billington S J SJ   Jost B H BH   Songer J G JG  

Journal of bacteriology 19980601 12


The 2.4-kb plasmid pAP1 from Arcanobacterium (Actinomyces) pyogenes had sequence similarity within the putative replication protein and double-stranded origin with the pIJ101/pJV1 family of plasmids. pJGS84, a derivative of pAP1 containing a kanamycin resistance gene, was able to replicate in Escherichia coli and Corynebacterium pseudotuberculosis, as well as in A. pyogenes. Detection of single-stranded DNA intermediates of pJGS84 replication suggested that this plasmid replicates by the rolling  ...[more]

Similar Datasets

| S-EPMC179514 | biostudies-other
| S-EPMC7103276 | biostudies-literature
| S-EPMC3147054 | biostudies-literature
| S-EPMC1563867 | biostudies-literature
| S-EPMC95193 | biostudies-literature
| S-EPMC126065 | biostudies-literature
| S-EPMC3792671 | biostudies-literature
| S-EPMC2665238 | biostudies-literature
| S-EPMC5775427 | biostudies-literature
| S-EPMC2519391 | biostudies-literature