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A high-performance polymer composite column for coronavirus nucleic acid purification.

ABSTRACT: Here, we report the development of a novel polymer composite (PC) purification column and kit. The performance of the PC columns was compared to conventional silica gel (SG) columns for the purification of nucleic acids from coronaviruses, including SARS-CoV-2, in 82 clinical samples. The results shows that PC-based purification outperforms silica gel (SG)-based purification by enabling a higher sensitivity (94%), accuracy (97%), and by eliminating false positives (100% specificity). The high specificity is critical for efficient patient triage and resource management during pandemics. Furthermore, PC-based purification exhibits three times higher analytical precision than a commonly used SG-based nucleic acid purification thereby enabling a more accurate quantification of viral loads and higher reproducibility.

SUBMITTER: Zarouri A

PROVIDER: S-EPMC10784286 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature

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A high-performance polymer composite column for coronavirus nucleic acid purification.

Zarouri Akli A Barnes Aaron M T AMT Aboubakr Hamada H Thekkudan Novi Vinni V Dong Qiuchen Q Nelson Andrew A Goyal Sagar S Abbas Abdennour A

Scientific reports 20240111 1

Here, we report the development of a novel polymer composite (PC) purification column and kit. The performance of the PC columns was compared to conventional silica gel (SG) columns for the purification of nucleic acids from coronaviruses, including SARS-CoV-2, in 82 clinical samples. The results shows that PC-based purification outperforms silica gel (SG)-based purification by enabling a higher sensitivity (94%), accuracy (97%), and by eliminating false positives (100% specificity). The high sp ...[more]

PMID: 38212439

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