Identification of homologous protein models via 3D comparisons using predicted structures.
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ABSTRACT:
SUBMITTER: Pan A
PROVIDER: S-EPMC10789644 | biostudies-literature | 2024 Jan
REPOSITORIES: biostudies-literature
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Identification of homologous protein models via 3D comparisons using predicted structures.
Pan Anyu A Shentu Jieyi J Zeng Yangfan Y Guo Rong R Yu Yang Y
STAR protocols 20240105 1
Recent advances in protein structure prediction enable 3D homology alignment and domain annotation using tertiary structures. Here, we present a protocol to identify homologous structures and annotate protein domains through in silico comparisons using the AlphaFold database. We describe steps for downloading and installing PyMOL software, preparing the query structure, and conducting a 3D homology search. The example provided highlights the application of this protocol in reevaluating an mpox v ...[more]
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