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Genome sequences of 31 mycobacteriophages isolated on Mycobacterium smegmatis mc²155 at room temperature.

ABSTRACT: We report the genome sequences of 31 mycobacteriophages isolated on Mycobacterium smegmatis mc²155 at room temperature. The genomes add to the diversity of Clusters A, B, C, G, and K. Collectively, the genomes include 70 novel protein-coding genes that have no close relatives among the actinobacteriophages.

SUBMITTER: Anders KR

PROVIDER: S-EPMC10793342 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature

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Genome sequences of 31 mycobacteriophages isolated on Mycobacterium smegmatis mc2155 at room temperature.

Anders Kirk R KR Abeyta Antonio A Andrade Christy C CC Bonilla Carla Y CY Braley Amanda B AB Bratt Alexandra G AG Duncan Kaya A KA Hayes Stephen G SG Robinson Ciara J CJ Smith-Flores Helen H Ettinger Ann-Scott H A-SH Ettinger William F WF Fay Marta M MM Haydock Joseph J McKenzie Sean K SK Garlena Rebecca A RA Russell Daniel A DA Poxleitner Marianne K MK

Microbiology resource announcements 20231215 1

We report the genome sequences of 31 mycobacteriophages isolated on Mycobacterium smegmatis mc2155 at room temperature. The genomes add to the diversity of Clusters A, B, C, G, and K. Collectively, the genomes include 70 novel protein-coding genes that have no close relatives among the actinobacteriophages. ...[more]

PMID: 38099681

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Project description:BackgroundPregnenolone and progesterone are the life-important steroid hormones regulating essential vital functions in mammals, and widely used in different fields of medicine. Microbiological production of these compounds from sterols is based on the use of recombinant strains expressing the enzyme system cholesterol hydroxylase/C20-C22 lyase (CH/L) of mammalian steroidogenesis. However, the efficiency of the known recombinant strains is still low. New recombinant strains and combination approaches are now needed to produce these steroid hormones.ResultsBased on Mycolicibacterium smegmatis, a recombinant strain was created that expresses the steroidogenesis system (CYP11A1, adrenodoxin reductase, adrenodoxin) of the bovine adrenal cortex. The recombinant strain transformed cholesterol and phytosterol to form progesterone among the metabolites. When 3-methoxymethyl ethers of sterols were applied as bioconversion substrates, the corresponding 3-ethers of pregnenolone and dehydroepiandrosterone (DHEA) were identified as major metabolites. Under optimized conditions, the recombinant strain produced 85.2 ± 4.7 mol % 3-methoxymethyl-pregnenolone within 48 h, while production of 3-substituted DHEA was not detected. After the 3-methoxymethyl function was deprotected by acid hydrolysis, crystalline pregnenolone was isolated in high purity (over 98%, w/w). The structures of steroids were confirmed using TLC, HPLC, MS and 1H- and 13C-NMR analyses.ConclusionThe use of mycolicybacteria as a microbial platform for the expression of systems at the initial stage of mammalian steroidogenesis ensures the production of valuable steroid hormones-progesterone and pregnenolone from cholesterol. Selective production of pregnenolone from cholesterol is ensured by the use of 3-substituted cholesterol as a substrate and optimization of the conditions for its bioconversion. The results open the prospects for the generation of the new microbial biocatalysts capable of effectively producing value-added steroid hormones.

Dataset Information

Genome sequences of 31 mycobacteriophages isolated on Mycobacterium smegmatis mc²155 at room temperature.

Publications

Genome sequences of 31 mycobacteriophages isolated on <i>Mycobacterium smegmatis</i> mc<sup>2</sup>155 at room temperature.

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