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Identification of Streptococcus canis isolated from milk of dairy cows with subclinical mastitis.


ABSTRACT: Streptococcus canis was isolated from 31 milk samples from 11 cows in a dairy herd (with 49 lactating cows) affected by subclinical mastitis in north Rhine-Westphalia, Germany. Thirty-one isolates from the infected udder quarters were further characterized for their phenotypic and molecular properties. Most isolates (83.9%) produced alpha-galactosidase, and all were negative for beta-d-glucuronidase. Amplification of the 16S rRNA gene by the PCR method and digestion with the restriction enzymes RsaI, MspI, and AvaII yielded species-specific patterns. Additional identification by species-specific amplification of the 16S rRNA gene, the 16S-23S rRNA gene intergenic spacer region, the CAMP factor-encoding gene cfg, and the internal fragments of the sodA gene was consistent with S. canis. Macrorestriction analysis of the chromosomal DNA by pulsed-field gel electrophoresis showed that the S. canis isolates originated from a single clone or were very closely related.

SUBMITTER: Hassan AA 

PROVIDER: S-EPMC1081216 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature

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Identification of Streptococcus canis isolated from milk of dairy cows with subclinical mastitis.

Hassan Abdulwahed Ahmed AA   Akineden Omer O   Usleber Ewald E  

Journal of clinical microbiology 20050301 3


Streptococcus canis was isolated from 31 milk samples from 11 cows in a dairy herd (with 49 lactating cows) affected by subclinical mastitis in north Rhine-Westphalia, Germany. Thirty-one isolates from the infected udder quarters were further characterized for their phenotypic and molecular properties. Most isolates (83.9%) produced alpha-galactosidase, and all were negative for beta-d-glucuronidase. Amplification of the 16S rRNA gene by the PCR method and digestion with the restriction enzymes  ...[more]

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