Dataset Information

Transcriptomic profiling of Dip2a in the neural differentiation of mouse embryonic stem cells.

ABSTRACT:

Introduction

The disconnected-interacting protein 2 homolog A (DIP2A), a member of disconnected-interacting 2 protein family, has been shown to be involved in human nervous system-related mental illness. This protein is highly expressed in the nervous system of mouse. Mutation of mouse DIP2A causes defects in spine morphology and synaptic transmission, autism-like behaviors, and defective social novelty [5], [27], indicating that DIP2A is critical to the maintenance of neural development. However, the role of DIP2A in neural differentiation has yet to be investigated.

Objective

To determine the role of DIP2A in neural differentiation, a neural differentiation model was established using mouse embryonic stem cells (mESCs) and studied by using gene-knockout technology and RNA-sequencing-based transcriptome analysis.

Results

We found that DIP2A is not required for mESCs pluripotency maintenance, but loss of DIP2A causes the neural differentiation abnormalities in both N2B27 and KSR medium. Functional knockout of Dip2a gene also decreased proliferation of mESCs by perturbation of the cell cycle and profoundly inhibited the expression of a large number of neural development-associated genes which mainly enriched in spinal cord development and postsynapse assembly.

Conclusions

The results of this report demonstrate that DIP2A plays an essential role in regulating differentiation of mESCs towards the neural fate.

SUBMITTER: Yao M

PROVIDER: S-EPMC10825174 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature

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Publications

Transcriptomic profiling of <i>Dip2a</i> in the neural differentiation of mouse embryonic stem cells.

Yao Mingze M Zhang Lei L Teng Xiaojuan X Lei Yu Y Xing Xiaoyu X Ren Tinglin T Pan Yuanqing Y Zhang Liwen L Li Zhengfeng Z Lin Jingxia J Zheng Yaowu Y Xing Li L Zhou Jiajian J Wu Changxin C

Computational and structural biotechnology journal 20231227

<h4>Introduction</h4>The disconnected-interacting protein 2 homolog A (DIP2A), a member of disconnected-interacting 2 protein family, has been shown to be involved in human nervous system-related mental illness. This protein is highly expressed in the nervous system of mouse. Mutation of mouse DIP2A causes defects in spine morphology and synaptic transmission, autism-like behaviors, and defective social novelty [5], [27], indicating that DIP2A is critical to the maintenance of neural development ...[more]

PMID: 38292475

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