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The murine SNF5/INI1 chromatin remodeling factor is essential for embryonic development and tumor suppression.

ABSTRACT: The assembly of eukaryotic DNA into nucleosomes and derived higher order structures constitutes a barrier for transcription, replication and repair. A number of chromatin remodeling complexes, as well as histone acetylation, were shown to facilitate gene activation. To investigate the function of two closely related mammalian SWI/SNF complexes in vivo, we inactivated the murine SNF5/INI1 gene, a common subunit of these two complexes. Mice lacking SNF5 protein stop developing at the peri-implantation stage, showing that the SWI/SNF complex is essential for early development and viability of early embryonic cells. Furthermore, heterozygous mice develop nervous system and soft tissue sarcomas. In these tumors the wild-type allele was lost, providing further evidence that SNF5 functions as a tumor suppressor gene in certain cell types.

SUBMITTER: Klochendler-Yeivin A

PROVIDER: S-EPMC1083796 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature

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The murine SNF5/INI1 chromatin remodeling factor is essential for embryonic development and tumor suppression.

Klochendler-Yeivin A A Fiette L L Barra J J Muchardt C C Babinet C C Yaniv M M

EMBO reports 20001201 6

The assembly of eukaryotic DNA into nucleosomes and derived higher order structures constitutes a barrier for transcription, replication and repair. A number of chromatin remodeling complexes, as well as histone acetylation, were shown to facilitate gene activation. To investigate the function of two closely related mammalian SWI/SNF complexes in vivo, we inactivated the murine SNF5/INI1 gene, a common subunit of these two complexes. Mice lacking SNF5 protein stop developing at the peri-implanta ...[more]

PMID: 11263494

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