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Turn-on fluorescent nanoprobe for ATP detection based on DNA-templated silver nanoclusters.

ABSTRACT: A turn-on fluorescence nanoprobe was constructed for the determination of adenosine 5'-triphosphate (ATP) based on DNA-templated silver nanoclusters (DNA-AgNCs). The significant enhancement fluorescence intensity of DNA-AgNCs in the presence of ATP is due to the high special binding affinity between ATP and the aptamer, resulting in the environment of DNA-AgNCs with darkish fluorescence lying at one terminus of DNA slightly altering owing to the change of ATP aptamer conformation. A good linear range runs from 9 to 24 mM with a satisfactory detection limit of 3 μM. Furthermore, the proposed nanoprobe exhibited good performance for ATP detection in diluted fetal bovine serum.

SUBMITTER: Li Y

PROVIDER: S-EPMC10863603 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Turn-on fluorescent nanoprobe for ATP detection based on DNA-templated silver nanoclusters.

Li Yuxia Y Meng Zeting Z Liu Yating Y Zhang Baozhu B

RSC advances 20240213 8

A turn-on fluorescence nanoprobe was constructed for the determination of adenosine 5'-triphosphate (ATP) based on DNA-templated silver nanoclusters (DNA-AgNCs). The significant enhancement fluorescence intensity of DNA-AgNCs in the presence of ATP is due to the high special binding affinity between ATP and the aptamer, resulting in the environment of DNA-AgNCs with darkish fluorescence lying at one terminus of DNA slightly altering owing to the change of ATP aptamer conformation. A good linear ...[more]

PMID: 38352688

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