Ontology highlight
ABSTRACT:
SUBMITTER: Dar SA
PROVIDER: S-EPMC1087575 | biostudies-literature | 2005 May
REPOSITORIES: biostudies-literature
Dar Shabir A SA Kuenen J Gijs JG Muyzer Gerard G
Applied and environmental microbiology 20050501 5
Here, we describe a three-step nested-PCR-denaturing gradient gel electrophoresis (DGGE) strategy to detect sulfate-reducing bacteria (SRB) in complex microbial communities from industrial bioreactors. In the first step, the nearly complete 16S rRNA gene was amplified using bacterial primers. Subsequently, this product was used as a template in a second PCR with group-specific SRB primers. A third round of amplification was conducted to obtain fragments suitable for DGGE. The largest number of b ...[more]