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Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis.

ABSTRACT: Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison.

SUBMITTER: Portillo MC

PROVIDER: S-EPMC3019698 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis.

Portillo M C MC Villahermosa D D Corzo A A Gonzalez J M JM

Applied and environmental microbiology 20101112 1

Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison. ...[more]

PMID: 21075891

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