Ontology highlight
ABSTRACT:
SUBMITTER: Sato A
PROVIDER: S-EPMC1087914 | biostudies-literature | 2005 Apr
REPOSITORIES: biostudies-literature
Sato Akitsugu A Nakada Kazuto K Akimoto Miho M Ishikawa Kaori K Ono Tomoko T Shitara Hiroshi H Yonekawa Hiromichi H Hayashi Jun-Ichi J
Proceedings of the National Academy of Sciences of the United States of America 20050413 17
The problem of whether recombinant mtDNAs are created in mammalian cells has been controversial for many years. We show convincing evidence for the very rare creation of recombinant mtDNA haplotypes by isolating human somatic hybrid cells and by generating mice carrying two different mtDNA haplotypes. To avoid misinterpretation of PCR-jumping products as recombinants, we used purified mtDNAs for cloning and sequencing. The results showed that only three of 318 clones of mtDNA purified from mouse ...[more]