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Heavy Metal Stabilization of DNA Origami Nanostructures.

ABSTRACT: DNA origami is a powerful tool to fold 3-dimensional DNA structures with nanometer precision. Its usage, however, is limited as high ionic strength, temperatures below ∼60 °C, and pH values between 5 and 10 are required to ensure the structural integrity of DNA origami nanostructures. Here, we demonstrate a simple and effective method to stabilize DNA origami nanostructures against harsh buffer conditions using [PdCl₄]^2-. It provided the stabilization of different DNA origami nanostructures against mechanical compression, temperatures up to 100 °C, double-distilled water, and pH values between 4 and 12. Additionally, DNA origami superstructures and bound cargos are stabilized with yields of up to 98%. To demonstrate the general applicability of our approach, we employed our protocol with a Pd metallization procedure at elevated temperatures. In the future, we think that our method opens up new possibilities for applications of DNA origami nanostructures beyond their usual reaction conditions.

SUBMITTER: Kemper U

PROVIDER: S-EPMC10905993 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Heavy Metal Stabilization of DNA Origami Nanostructures.

Kemper Ulrich U Weizenmann Nicole N Kielar Charlotte C Erbe Artur A Seidel Ralf R

Nano letters 20240216 8

DNA origami is a powerful tool to fold 3-dimensional DNA structures with nanometer precision. Its usage, however, is limited as high ionic strength, temperatures below ∼60 °C, and pH values between 5 and 10 are required to ensure the structural integrity of DNA origami nanostructures. Here, we demonstrate a simple and effective method to stabilize DNA origami nanostructures against harsh buffer conditions using [PdCl<sub>4</sub>]<sup>2-</sup>. It provided the stabilization of different DNA origa ...[more]

PMID: 38363878

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