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Fluorous-Directed Assembly of DNA Origami Nanostructures.

ABSTRACT: An orthogonal, noncovalent approach to direct the assembly of higher-order DNA origami nanostructures is described. By incorporating perfluorinated tags into the edges of DNA origami tiles we control their hierarchical assembly via fluorous-directed recognition. When we combine this approach with Watson-Crick base-pairing we form discrete dimeric constructs in significantly higher yield (8x) than when either molecular recognition method is used in isolation. This integrated "catch-and-latch" approach, which combines the strength and mobility of the fluorous effect with the specificity of base-pairing, provides an additional toolset for DNA nanotechnology, one that enables increased assembly efficiency while requiring significantly fewer DNA sequences. As a result, our integration of fluorous-directed assembly into origami systems represents a cheap, atom-efficient means to produce discrete superstructures.

SUBMITTER: Zou J

PROVIDER: S-EPMC9835977 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Fluorous-Directed Assembly of DNA Origami Nanostructures.

Zou Jiajia J Stammers Ashley C AC Taladriz-Sender Andrea A Withers Jamie M JM Christie Iain I Santana Vega Marina M Aekbote Badri L BL Peveler William J WJ Rusling David A DA Burley Glenn A GA Clark Alasdair W AW

ACS nano 20221220 1

An orthogonal, noncovalent approach to direct the assembly of higher-order DNA origami nanostructures is described. By incorporating perfluorinated tags into the edges of DNA origami tiles we control their hierarchical assembly via fluorous-directed recognition. When we combine this approach with Watson-Crick base-pairing we form discrete dimeric constructs in significantly higher yield (8x) than when either molecular recognition method is used in isolation. This integrated "catch-and-latch" app ...[more]

PMID: 36537902

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