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Loss of mitochondrial DNA is associated with reduced DNA content variability in Saccharomyces cerevisiae.

ABSTRACT: DNA content measurement by fluorescence-assisted cell sorting (FACS) provides information on cell cycle progression and DNA content variability. Saccharomyces cerevisiae mutants with DNA content variability that was reduced relative to wild-type strains had defects in mitochondrial DNA (mtDNA) maintenance and mitochondrial gene expression and were correlated with strains found to lack mtDNA ([ rho ⁰ ] cells) by genome sequencing and fluorescence microscopy. In contrast, mutants with increased variability had defects in cell cycle progression, which may indicate a loss of coordination between mtDNA and nuclear DNA replication. Thus, FACS measurement of DNA content variability can provide insight into cell-to-cell heterogeneity in mtDNA copy number.

SUBMITTER: Putnam CD

PROVIDER: S-EPMC10964099 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature

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Loss of mitochondrial DNA is associated with reduced DNA content variability in Saccharomyces cerevisiae.

Putnam Christopher D CD

microPublication biology 20240311

DNA content measurement by fluorescence-assisted cell sorting (FACS) provides information on cell cycle progression and DNA content variability. Saccharomyces cerevisiae mutants with DNA content variability that was reduced relative to wild-type strains had defects in mitochondrial DNA (mtDNA) maintenance and mitochondrial gene expression and were correlated with strains found to lack mtDNA ([ rho 0 ] cells) by genome sequencing and fluorescence microscopy. In contrast, ...[more]

PMID: 38533353

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