Ontology highlight
ABSTRACT:
SUBMITTER: Grummitt AR
PROVIDER: S-EPMC1133823 | biostudies-literature | 2004 Sep
REPOSITORIES: biostudies-literature
Grummitt Annaleise R AR Rutledge Peter J PJ Clifton Ian J IJ Baldwin Jack E JE
The Biochemical journal 20040901 Pt 2
Isopenicillin N synthase (IPNS) is a non-haem iron oxidase that catalyses the formation of bicyclic isopenicillin N from delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV). In this study we report a novel activity for the iron of the IPNS active site, which behaves as a Lewis acid to catalyse the elimination of HF from the fluorinated substrate analogue, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-beta-fluorovaline (ACbetaFV). X-Ray crystallographic studies of IPNS crystals grown anaerobical ...[more]