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Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibility.

ABSTRACT: The role of Aspergillus fumigatus 14alpha-sterol demethylase (Cyp51A) in azole drug susceptibility was assessed. Targeted disruption of cyp51A in azole-susceptible and -resistant strains decreased MICs from 2- to 40-fold. The cyp51A mutants were morphologically indistinguishable from the wild-type strain, retaining the ability to cause pulmonary disease in neutropenic mice.

SUBMITTER: Mellado E 

PROVIDER: S-EPMC1140498 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature

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Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibility.

Mellado E E   Garcia-Effron G G   Buitrago M J MJ   Alcazar-Fuoli L L   Cuenca-Estrella M M   Rodriguez-Tudela J L JL  

Antimicrobial agents and chemotherapy 20050601 6

The role of Aspergillus fumigatus 14alpha-sterol demethylase (Cyp51A) in azole drug susceptibility was assessed. Targeted disruption of cyp51A in azole-susceptible and -resistant strains decreased MICs from 2- to 40-fold. The cyp51A mutants were morphologically indistinguishable from the wild-type strain, retaining the ability to cause pulmonary disease in neutropenic mice. ...[more]

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