Ontology highlight
ABSTRACT:
SUBMITTER: Vega-Arreguin JC
PROVIDER: S-EPMC1143747 | biostudies-literature | 2005 Jul
REPOSITORIES: biostudies-literature
Vega-Arreguín Julio C JC Timchenko Tatiana T Gronenborn Bruno B Ramírez Bertha Cecilia BC
Journal of virology 20050701 13
Replication initiation of nanoviruses, plant viruses with a multipartite circular single-stranded DNA genome, is triggered by the master Rep (M-Rep) protein. To enable the study of interactions between M-Rep and viral or host factors involved in replication, we designed oligohistidine-tagged variants of the nanovirus Faba bean necrotic yellows virus (FBNYV) M-Rep protein that allow affinity purification of enzymatically active M-Rep from plant tissue. The tagged M-Rep protein was able to initiat ...[more]