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Identification of the regions of Fv1 necessary for murine leukemia virus restriction.

ABSTRACT: The Fv1 gene restricts murine leukemia virus replication via an interaction with the viral capsid protein. To study this interaction, a number of mutations, including a series of N-terminal and C-terminal deletions, internal deletions, and a number of single-amino-acid substitutions, were introduced into the n and b alleles of the Fv1 gene and the effects of these changes on virus restriction were measured. A significant fraction of the Fv1 protein was not required for restriction; however, retention of an intact major homology region as well as of domains toward the N and C termini was essential. Binding specificity appeared to be a combinatorial property of a number of residues within the C-terminal portion of Fv1.

SUBMITTER: Bishop KN

PROVIDER: S-EPMC114923 | biostudies-literature | 2001 Jun

REPOSITORIES: biostudies-literature

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Identification of the regions of Fv1 necessary for murine leukemia virus restriction.

Bishop K N KN Bock M M Towers G G Stoye J P JP

Journal of virology 20010601 11

The Fv1 gene restricts murine leukemia virus replication via an interaction with the viral capsid protein. To study this interaction, a number of mutations, including a series of N-terminal and C-terminal deletions, internal deletions, and a number of single-amino-acid substitutions, were introduced into the n and b alleles of the Fv1 gene and the effects of these changes on virus restriction were measured. A significant fraction of the Fv1 protein was not required for restriction; however, rete ...[more]

PMID: 11333899

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