Large-scale engineering of the Corynebacterium glutamicum genome.
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ABSTRACT:
SUBMITTER: Suzuki N
PROVIDER: S-EPMC1151864 | biostudies-literature | 2005 Jun
REPOSITORIES: biostudies-literature
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Large-scale engineering of the Corynebacterium glutamicum genome.
Suzuki Nobuaki N Okayama Satoshi S Nonaka Hiroshi H Tsuge Yota Y Inui Masayuki M Yukawa Hideaki H
Applied and environmental microbiology 20050601 6
The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. To construct an improved C. glutamicum genome, we developed a precise genome excision method based on the Cre/loxP recombination system and successfully deleted 11 distinct genomic regions identified by comparative analysis of C. glutamicum genomes. Despite the loss of several predicted open reading frames, the mutant cells exhibited normal growth under standard laboratory conditions. With a total ...[more]
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