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Use of the genomic subtractive hybridization technique to develop a real-time PCR assay for quantitative detection of Prevotella spp. in oral biofilm samples.

ABSTRACT: Genomic subtractive hybridization was used to design Prevotella nigrescens-specific primers and TaqMan probes. Based on this technique, a TaqMan real-time PCR assay was developed for quantifying four oral black-pigmented Prevotella species. The combination of real-time PCR and genomic subtractive hybridization is useful for preparing species-specific primer-probe sets for closely related species.

SUBMITTER: Nagashima S

PROVIDER: S-EPMC1151961 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature

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Use of the genomic subtractive hybridization technique to develop a real-time PCR assay for quantitative detection of Prevotella spp. in oral biofilm samples.

Nagashima Shiori S Yoshida Akihiro A Suzuki Nao N Ansai Toshihiro T Takehara Tadamichi T

Journal of clinical microbiology 20050601 6

Genomic subtractive hybridization was used to design Prevotella nigrescens-specific primers and TaqMan probes. Based on this technique, a TaqMan real-time PCR assay was developed for quantifying four oral black-pigmented Prevotella species. The combination of real-time PCR and genomic subtractive hybridization is useful for preparing species-specific primer-probe sets for closely related species. ...[more]

PMID: 15956428

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