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Genomic analysis of heat-shock factor targets in Drosophila.

ABSTRACT: We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.

SUBMITTER: Birch-Machin I

PROVIDER: S-EPMC1175994 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature

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Genomic analysis of heat-shock factor targets in Drosophila.

Birch-Machin Ian I Gao Shan S Huen David D McGirr Richard R White Robert A H RA Russell Steven S

Genome biology 20050610 7

We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using inta ...[more]

PMID: 15998452

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