Ontology highlight
ABSTRACT:
SUBMITTER: Kohn JE
PROVIDER: S-EPMC1182433 | biostudies-literature | 2005 Aug
REPOSITORIES: biostudies-literature
Kohn Jonathan E JE Plaxco Kevin W KW
Proceedings of the National Academy of Sciences of the United States of America 20050726 31
Hybridization-induced conformational changes have been successfully used in biosensors for the transduction of DNA-binding events into readily observable optical or electronic signals. Similar signal transduction has not, however, proven of equal utility in protein-based biosensors. The discrepancy arises because, unlike ssDNA, most proteins do not undergo significant conformational changes upon ligand binding. Here, we describe a solution to this problem. We show that an arbitrarily selected, n ...[more]