Dataset Information

Differentially expressed genes in association with in vitro invasiveness of human epithelioid sarcoma.

ABSTRACT:

Aims

Differential display reverse transcription polymerase chain reaction (RT-PCR) was performed to identify genes associated with the invasive potential of human epithelioid sarcoma.

Methods

Two different clonal subpopulations, GRU-1A and GRU-1B, derived from the same human epithelioid sarcoma cell line GRU-1 and known to differ greatly in their invasive potential were compared by means of mRNA fingerprinting.

Results

Using a set of 10 arbitrary upstream primers and nine anchored oligo-dT primers, 22 candidate gene fragments were identified; differential expression was confirmed in four of these fragments by northern blot analysis. At the mRNA level, apoferritin light chain was predominantly expressed by the highly invasive cell line GRU-1A. In contrast, the mitochondrial gene M1, encoding cytochrome c oxidase I, and the TI-227H gene were expressed more strongly by the low invasive cell line GRU-1B. Furthermore, a novel gene fragment was identified and cloned that was preferentially expressed in the low invasive cell line GRU-1B, and therefore might have an inhibitory role in invasion. Consequently, this gene fragment was designated as expressed in low invasive sarcoma cells (ELISC-1).

Conclusions

A novel gene fragment (ELISC-1) and three known genes were identified as potential regulators of tumour invasiveness. Cloning of the entire sequence of ELISC-1 and subsequent investigations are required to establish its biological role.

SUBMITTER: Weber A

PROVIDER: S-EPMC1187090 | biostudies-literature | 2001 Oct

REPOSITORIES: biostudies-literature

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Publications

Differentially expressed genes in association with in vitro invasiveness of human epithelioid sarcoma.

Weber A A Engers R R Nockemann S S Gohr L L LL Zur Hausen A A Gabbert H E HE

Molecular pathology : MP 20011001 5

<h4>Aims</h4>Differential display reverse transcription polymerase chain reaction (RT-PCR) was performed to identify genes associated with the invasive potential of human epithelioid sarcoma.<h4>Methods</h4>Two different clonal subpopulations, GRU-1A and GRU-1B, derived from the same human epithelioid sarcoma cell line GRU-1 and known to differ greatly in their invasive potential were compared by means of mRNA fingerprinting.<h4>Results</h4>Using a set of 10 arbitrary upstream primers and nine a ...[more]

PMID: 11577175

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Dataset Information

Differentially expressed genes in association with in vitro invasiveness of human epithelioid sarcoma.

Aims

Methods

Results

Conclusions

Publications

Differentially expressed genes in association with in vitro invasiveness of human epithelioid sarcoma.

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