Ontology highlight
ABSTRACT:
SUBMITTER: Jidenko M
PROVIDER: S-EPMC1187984 | biostudies-literature | 2005 Aug
REPOSITORIES: biostudies-literature
Jidenko Marie M Nielsen Rikke C RC Sørensen Thomas Lykke-Møller TL Møller Jesper V JV le Maire Marc M Nissen Poul P Jaxel Christine C
Proceedings of the National Academy of Sciences of the United States of America 20050808 33
The Ca2+-ATPase SERCA1a (sarcoplasmic-endoplasmic reticulum Ca2+-ATPase isoform 1a) from rabbit has been overexpressed in Saccharomyces cerevisiae. This membrane protein was purified by avidin agarose affinity chromatography based on natural biotinylation in the expression host, followed by HPLC gel filtration. Both the functional and structural properties of the overexpressed protein validate the method. Thus, calcium-dependent ATPase activity and calcium transport are essentially intact after ...[more]