Mapping of the binding site for Mannheimia haemolytica leukotoxin within bovine CD18.
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ABSTRACT:
SUBMITTER: Dileepan T
PROVIDER: S-EPMC1201247 | biostudies-literature | 2005 Aug
REPOSITORIES: biostudies-literature
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Mapping of the binding site for Mannheimia haemolytica leukotoxin within bovine CD18.
Dileepan T T Kannan M S MS Walcheck B B Thumbikat P P Maheswaran S K SK
Infection and immunity 20050801 8
To map the site involved in Mannheimia haemolytica leukotoxin (LktA) binding and biological activity within bovine CD18, bovine x human CD18 chimeric constructs were generated and coexpressed with bovine CD11a in K562 cells. Studies with the chimeric leukocyte function-associated antigen 1 transductants demonstrate that the site required for LktA binding and biological effects resides within amino acid residues 500 and 600 of the extracellular region of bovine CD18. ...[more]
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