Mannheimia (Pasteurella) haemolytica leukotoxin binding domain lies within amino acids 1 to 291 of bovine CD18.
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ABSTRACT:
SUBMITTER: Gopinath RS
PROVIDER: S-EPMC1231049 | biostudies-literature | 2005 Sep
REPOSITORIES: biostudies-literature
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Mannheimia (Pasteurella) haemolytica leukotoxin binding domain lies within amino acids 1 to 291 of bovine CD18.
Gopinath R S RS Ambagala T C TC Deshpande M S MS Donis R O RO Srikumaran S S
Infection and immunity 20050901 9
Previously, we identified bovine CD18 as the receptor for leukotoxin secreted by Mannheimia (Pasteurella) haemolytica. In this study, we constructed bovine-murine CD18 chimeras to locate the leukotoxin binding domain on CD18. Leukotoxin specifically lysed transfectants expressing bovine CD18 fragment encompassing amino acids 1 to 291, indicating that leukotoxin binding domain lies within amino acids 1 to 291 of bovine CD18. ...[more]
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