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Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: differential binding of P and M helical enantiomers.

ABSTRACT: We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 10(7) M(-1) and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer.

SUBMITTER: Meistermann I

PROVIDER: S-EPMC122723 | biostudies-literature | 2002 Apr

REPOSITORIES: biostudies-literature

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Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: differential binding of P and M helical enantiomers.

Meistermann Isabelle I Moreno Virtudes V Prieto Maria J MJ Moldrheim Erlend E Sletten Einar E Khalid Syma S Rodger P Mark PM Peberdy Jemma C JC Isaac Christian J CJ Rodger Alison A Hannon Michael J MJ

Proceedings of the National Academy of Sciences of the United States of America 20020401 8

We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 10(7) M(-1) and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coilin ...[more]

PMID: 11959957

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