Expressible molecular colonies.
Ontology highlight
ABSTRACT:
SUBMITTER: Samatov TR
PROVIDER: S-EPMC1243806 | biostudies-literature | 2005
REPOSITORIES: biostudies-literature
Publications
Expressible molecular colonies.
Samatov Timur R TR Chetverina Helena V HV Chetverin Alexander B AB
Nucleic acids research 20051004 17
Carrying out polymerase chain reaction in a gel layer generates a 2-D pattern of DNA colonies comprising pure genetic clones. Here we demonstrate that transcription, translation and protein folding can be performed in the same gel. The resulting nucleoprotein colonies mimic living cells by serving as compartments in which the synthesized RNAs and proteins co-localize with their templates. Yet, due to the absence of penetration barriers, such a molecular colony display allows cloned genes to be d ...[more]
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