Ontology highlight
ABSTRACT:
SUBMITTER: Flood DT
PROVIDER: S-EPMC7923357 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Flood Dillon T DT Hintzen Jordi C J JCJ Knouse Kyle W KW Hill David E DE Lu Chenxi C Cistrone Philip A PA Chen Jason S JS Otomo Takanori T Dawson Philip E PE
Proceedings of the National Academy of Sciences of the United States of America 20210201 8
Site-selective chemical bioconjugation reactions are enabling tools for the chemical biologist. Guided by a careful study of the selenomethionine (SeM) benzylation, we have refined the reaction to meet the requirements of practical protein bioconjugation. SeM is readily introduced through auxotrophic expression and exhibits unique nucleophilic properties that allow it to be selectively modified even in the presence of cysteine. The resulting benzylselenonium adduct is stable at physiological pH, ...[more]