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P600, a unique protein required for membrane morphogenesis and cell survival.


ABSTRACT: In this article, we identify and characterize p600, a unique 600-kDa retinoblastoma protein- and calmodulin-binding protein. In the nucleus, p600 and retinoblastoma protein seem to act as a chromatin scaffold. In the cytoplasm, p600 and clathrin form a meshwork structure, which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interference RNA abrogates integrin-mediated ruffled membrane formation and, furthermore, prevents activation of integrin-mediated survival pathways. Consequently, knockdown of p600 sensitizes cells to apoptosis induced by cell detachment. These findings provide mechanistic insight into the regulation of membrane-proximal events in tumorigenesis.

SUBMITTER: Nakatani Y 

PROVIDER: S-EPMC1247991 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature

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p600, a unique protein required for membrane morphogenesis and cell survival.

Nakatani Yoshihiro Y   Konishi Hiroaki H   Vassilev Alex A   Kurooka Hisanori H   Ishiguro Keiichiro K   Sawada Jun-ichi J   Ikura Tsuyoshi T   Korsmeyer Stanley J SJ   Qin Jun J   Herlitz Anna M AM  

Proceedings of the National Academy of Sciences of the United States of America 20051007 42


In this article, we identify and characterize p600, a unique 600-kDa retinoblastoma protein- and calmodulin-binding protein. In the nucleus, p600 and retinoblastoma protein seem to act as a chromatin scaffold. In the cytoplasm, p600 and clathrin form a meshwork structure, which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interference RNA abrogates integrin-mediated ruffled membrane formation and, furthermore, prevents activation of in  ...[more]

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