Ontology highlight
ABSTRACT:
SUBMITTER: Nairz K
PROVIDER: S-EPMC124979 | biostudies-literature | 2002 Aug
REPOSITORIES: biostudies-literature
Nairz Knud K Stocker Hugo H Schindelholz Benno B Hafen Ernst E
Proceedings of the National Academy of Sciences of the United States of America 20020729 16
With the availability of complete genome sequences, new rapid and reliable strategies for positional cloning become possible. Single-nucleotide polymorphisms (SNPs) permit the mapping of mutations at a resolution not amenable to classical genetics. Here we describe a SNP mapping procedure that relies on resolving polymorphisms by denaturing HPLC without the necessity of determining the nature of the SNPs. With the example of mapping mutations to the Drosophila nicastrin locus, we discuss the ben ...[more]