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Global analysis of stress-regulated mRNA turnover by using cDNA arrays.

ABSTRACT: cDNA array technology has proven to be a powerful way to monitor global changes in gene expression patterns. Here, we present an approach that extends the current utility of cDNA arrays to allow the evaluation of the relative roles of transcription and mRNA turnover in governing gene expression on a global basis, compared with current individual gene-by-gene analyses. This method, which involves comparison of large-scale hybridization patterns generated with steady-state mRNA versus newly transcribed (nuclear run-on) RNA, was used to demonstrate the importance of mRNA turnover in regulating gene expression following several conditions of stress.

SUBMITTER: Fan J 

PROVIDER: S-EPMC124989 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature

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Global analysis of stress-regulated mRNA turnover by using cDNA arrays.

Fan Jinshui J   Yang Xiaoling X   Wang Wengong W   Wood William H WH   Becker Kevin G KG   Gorospe Myriam M  

Proceedings of the National Academy of Sciences of the United States of America 20020729 16

cDNA array technology has proven to be a powerful way to monitor global changes in gene expression patterns. Here, we present an approach that extends the current utility of cDNA arrays to allow the evaluation of the relative roles of transcription and mRNA turnover in governing gene expression on a global basis, compared with current individual gene-by-gene analyses. This method, which involves comparison of large-scale hybridization patterns generated with steady-state mRNA versus newly transc  ...[more]