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Translocation portals for the substrates and products of a viral transcription complex: the bluetongue virus core.

ABSTRACT: The bluetongue virus core is a molecular machine that simultaneously and repeatedly transcribes mRNA from 10 segments of viral double-stranded RNA, packaged in a liquid crystalline array. To determine how the logistical problems of transcription within a sealed shell are solved, core crystals were soaked with various ligands and analysed by X-ray crystallography. Mg(2+) ions produce a slight expansion of the capsid around the 5-fold axes. Oligonucleotide soaks demonstrate that the 5-fold pore, opened up by this expansion, is the exit site for mRNA, whilst nucleotide soaks pinpoint a separate binding site that appears to be a selective channel for the entry and exit of substrates and by-products. Finally, nucleotides also bind to the outer core layer, providing a substrate sink.

SUBMITTER: Diprose JM

PROVIDER: S-EPMC125797 | biostudies-literature | 2001 Dec

REPOSITORIES: biostudies-literature

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Translocation portals for the substrates and products of a viral transcription complex: the bluetongue virus core.

Diprose J M JM Burroughs J N JN Sutton G C GC Goldsmith A A Gouet P P Malby R R Overton I I Ziéntara S S Mertens P P PP Stuart D I DI Grimes J M JM

The EMBO journal 20011201 24

The bluetongue virus core is a molecular machine that simultaneously and repeatedly transcribes mRNA from 10 segments of viral double-stranded RNA, packaged in a liquid crystalline array. To determine how the logistical problems of transcription within a sealed shell are solved, core crystals were soaked with various ligands and analysed by X-ray crystallography. Mg(2+) ions produce a slight expansion of the capsid around the 5-fold axes. Oligonucleotide soaks demonstrate that the 5-fold pore, o ...[more]

PMID: 11742999

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