Ontology highlight
ABSTRACT:
SUBMITTER: Braddock DT
PROVIDER: S-EPMC126100 | biostudies-literature | 2002 Jul
REPOSITORIES: biostudies-literature
Braddock Demetrios T DT Baber James L JL Levens David D Clore G Marius GM
The EMBO journal 20020701 13
To elucidate the basis of sequence-specific single-stranded (ss) DNA recognition by K homology (KH) domains, we have solved the solution structure of a complex between the KH3 domain of the transcriptional regulator heterogeneous nuclear ribonucleoprotein K (hnRNP K) and a 10mer ssDNA. We show that hnRNP K KH3 specifically recognizes a tetrad of sequence 5'd-TCCC. The complex is stabilized by a dense network of methyl-oxygen hydrogen bonds involving the methyl groups of three isoleucine residues ...[more]