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Global role of the protein kinase Gcn2 in the human pathogen Candida albicans.


ABSTRACT: The pathogen Candida albicans responds to amino acid starvation by activating pseudohyphal development and the expression of amino acid biosynthetic genes (GCN response). In Saccharomyces cerevisiae, the GCN response is dependent on Gcn2, which regulates the translation of the transcription factor Gcn4. Therefore, we examined the role of Gcn2 in C. albicans by using molecular, cellular, and genomic approaches. We show that C. albicans GCN2 encodes an eIF2alpha kinase, like its S. cerevisiae homologue. However, GCN4 appears to be regulated mainly at the transcriptional level in C. albicans. Furthermore, the inactivation of C. albicans Gcn2 only partially attenuates growth under amino acid starvation conditions and resistance to the histidine analogue 3-aminotriazole. Our comparison of the Gcn4 and Gcn2 regulons by transcript profiling reinforces the view that Gcn2 contributes to, but is not essential for, the activation of general amino acid control in C. albicans.

SUBMITTER: Tournu H 

PROVIDER: S-EPMC1265895 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature

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Global role of the protein kinase Gcn2 in the human pathogen Candida albicans.

Tournu Hélène H   Tripathi Gyanendra G   Bertram Gwyneth G   Macaskill Susan S   Mavor Abigail A   Walker Louise L   Odds Frank C FC   Gow Neil A R NA   Brown Alistair J P AJ  

Eukaryotic cell 20051001 10


The pathogen Candida albicans responds to amino acid starvation by activating pseudohyphal development and the expression of amino acid biosynthetic genes (GCN response). In Saccharomyces cerevisiae, the GCN response is dependent on Gcn2, which regulates the translation of the transcription factor Gcn4. Therefore, we examined the role of Gcn2 in C. albicans by using molecular, cellular, and genomic approaches. We show that C. albicans GCN2 encodes an eIF2alpha kinase, like its S. cerevisiae homo  ...[more]

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